Comparison of different chromatin staining techniques for bull sperm

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Comparison of different chromatin staining techniques for bull sperm

Morphological analysis of semen is a very important step in fertility assessment, but many semen defects are not detectable at the morphological level. These include pathological changes in sperm chromatin structure. During mammalian spermiogenesis, histone proteins associated with DNA structure are replaced by specific protamines, with which chromatin does not form nucleosomal complexes. In th...

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Evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. Routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear DNA integrity of spermatozoa. It has been suggested that altered nuclear chromatin struc...

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ژورنال

عنوان ژورنال: Archives Animal Breeding

سال: 2014

ISSN: 2363-9822

DOI: 10.7482/0003-9438-57-013